LIMA™ is a hyperspectral microscope based on a widely tunable laser source. It uses Photon etc.’s filtering technology to illuminate the full field of view of a research-grade microscope with a continuously tunable monochromatic, high power density laser light. The system provides a high spectral resolution in the VIS, NIR, and SWIR combined to a near-diffraction-limited spatial resolution. Ideal for darkfield, PLE, or standard brightfield reflectance and transmittance imaging, it can map the full spectral response of a sample much faster than point-by-point or line-scan-based systems.
VIS | SWIR | eSWIR | VIS-SWIR | |
---|---|---|---|---|
Excitation spectral range | 400 - 1000 nm | 900 - 1620 nm | 1000 - 2500 nm | 400 - 1620 nm |
Excitation spectral resolution (FWHM) | 1.5 - 2.5 nm | 3.0 - 5.0 nm | < 5.0 nm | 1.5 - 5.0 nm |
Excitation wavelength | Continuously tunable | Continuously tunable | Continuously tunable | Continuously tunable |
Out-of-band rejection | < -60 dB @ ± 40 nm | < -60 dB @ ± 80 nm | < -60 dB @ ± 80 nm | < -60 dB @ ± 40 nm (VIS) or 80 nm (SWIR) |
TLS output power | 2 - 4 mW | 3 - 9 mW | 0.5 - 8.0 mW | 2 - 9 mW |
Illumination | High efficiency homogeneous illumination; Dia or Epi illumination; Brightfield; Darkfield (oil and dry) | High efficiency homogeneous illumination; Dia or Epi illumination; Brightfield; Darkfield (oil and dry) | High efficiency homogeneous illumination; Dia or Epi illumination; Brightfield; Darkfield (oil and dry) | High efficiency homogeneous illumination; Dia or Epi illumination; Brightfield; Darkfield (oil and dry) |
Spatial resolution | sub-micron - Limited by the microscope objective NA | sub-micron - Limited by the microscope objective NA | sub-micron - Limited by the microscope objective NA | sub-micron - Limited by the microscope objective NA |
Camera | sCMOS (optionally CCD, EMCCD) | ZephIR™ 1.7 or Alizé™ 1.7 (InGaAs) | ZephIR™ 2.5 (HgCdTe) | sCMOS and ZephIR™ 1.7 or Alizé™ 1.7 |
Microscope | Upright or inverted, scientific grade | Upright or inverted, scientific grade | Upright or inverted, scientific grade | Upright or inverted, scientific grade |
White light illumination | Diascopic, episcopic, Hg, halogen | Diascopic, episcopic, Hg, halogen | Diascopic, episcopic, Hg, halogen | Diascopic, episcopic, Hg, halogen |